Evaluation Of Single Stage Hybrid Deammonification Reactor For Filtrate Treatment: Start-Up And Performance

In this manuscript, we report the startup and operation of suspended growth and hybrid (i.e IFAS type) deammonification reactors. Both reactors were started with biomass from Rock River, Virginia Pilot Scale single stage deammonification reactor. Both reactors were fed with real centrate (reject water from the anaerobic digester) collected from a local wastewater treatment plant. The overall nitrogen loading rate to the hybrid reactor was 84.8 mg N/L-day with a maximum of 91.7 mg N/L-day. The average ammonium concentration was 591.8 mg/L in the influent and 121.8 mg/L in the effluent, resulting in an overall ammonium removal efficiency of 79%. The suspended growth reactor also performed equally well with nitritein the final effluent always below 1 mg/L. The whole metagenomic analysis revealed that the anammox suspended growth reactor was comparatively more enriched in phylum Planctomycetes, of Anammox bacteria. Based on Ribosomal database it was found that both the reactors were enriched in genus Candidatus Brocadia OUT 2413, Methyloversatilis unclassified OUT 3158, Chlorobi SJA-28 OTU 1309. Anammox suspended growth reactor was also found to harbor a unique group of Anammox bacteria, clone KIST-JJ028 Unclassified OUT 2959