Rapid Detection of Bacteria in Water by Quantum Dots Labeling and Magnetic Separation

Shuping Li; Zhongxian Guo

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Rapid Detection of Bacteria in Water by Quantum Dots Labeling and Magnetic Separation

Abstract

Wheat germ agglutinin functionalized CdSe-ZnS quantum dots (WGA-QDs) were utilized as the fluorescent label for the bacterial detection. The labeled bacteria cells were enriched and separated with SiMAG-PEI, a type of non-porous silica-encapsulated Fe3O4 magnetic particles with anion-exchange group. The study indicated that the bacterial cells interact strongly with WGA-QDs and SiMAG-PEI and the fluorescence intensity increases with increasing bacterial concentration within the range of 102–107 cfu/ml. This method can be used to detect total bacterial concentration in about 1.5 hour.

Wheat germ agglutinin functionalized CdSe-ZnS quantum dots (WGA-QDs) were utilized as the fluorescent label for the bacterial detection. The labeled bacteria cells were enriched and separated with SiMAG-PEI, a type of non-porous silica-encapsulated Fe3O4 magnetic particles with anion-exchange group. The study indicated that the bacterial cells interact strongly with WGA-QDs and SiMAG-PEI and the...

Author(s)

Shuping LiZhongxian Guo

SourceProceedings of the Water Environment Federation

SubjectSession 105: Detecting and Control of Sponges, Algae, and Bacteria

Document typeConference Paper

PublisherWater Environment Federation

Print publication date Jan, 2011

ISSN1938-6478

SICI1938-6478(20110101)2011:8L.6729;1-

DOI10.2175/193864711802793416

Volume / Issue2011 / 8

Content sourceWEFTEC