This page cannot be printed from here

Please use the dedicated print option from the 'view' drop down menu located in the blue ribbon in the top, right section of the publication.

screenshot of print menu option

Description: Book cover
Identification of Thermodynamically Inaccessible Target Sites for Fluorescence In Situ Hybridization (FISH)
Abstract
Understanding accessibility of the target site in fluorescence in situ hybridization (FISH) is critical for predicting the efficiency of hybridization. In this study, we investigated whether the free energy related to the unfolding of the target molecule (δG03) was a good predictor of target site accessibility. Using a theoretical analysis we identified potential problematic sites based on a generated δG03 map and experimentally studied one of those sites in detail. It was shown that although δG03 was a useful parameter for predicting accessibility, there was also a need for defining its limitations.
Understanding accessibility of the target site in fluorescence in situ hybridization (FISH) is critical for predicting the efficiency of hybridization. In this study, we investigated whether the free energy related to the unfolding of the target molecule (δG03) was a good predictor of target site accessibility. Using a theoretical analysis we identified potential problematic sites based on a...
Author(s)
Hatice E. OktenL. Safak YilmazDaniel R. Noguera
SourceProceedings of the Water Environment Federation
SubjectSession 64: Practice-Based Application of Molecular Tools
Document typeConference Paper
PublisherWater Environment Federation
Print publication date Jan, 2007
ISSN1938-6478
SICI1938-6478(20070101)2007:13L.5010;1-
DOI10.2175/193864707787969540
Volume / Issue2007 / 13
Content sourceWEFTEC
First / last page(s)5010 - 5019
Copyright2007
Word count104

Purchase price $11.50

Get access