Next-Generation DNA Sequencing Identifies Pathogens in Biosolids

Stabilized sewage sludge, biosolids, is often land applied as a fertilizer replacement. It is currently unclear what risk this poses to residents living near land application sites due to the potential pathogen content in biosolids. Next generation DNA sequencing was utilized to provide an unbiased view of bacterial and viral pathogen diversity. This sequencing effort was successful at identifying both bacterial and viral pathogens and suggests that DNA sequencing is useful as a preliminary tool in directing pathogen survey and monitoring efforts.